Coding

Part:BBa_K4367010:Design

Designed by: Anders Kllberg   Group: iGEM22_Chalmers-Gothenburg   (2022-10-11)


Tobacco Etch Virus Protease (TEVp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 799
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 806


Design Notes

It's important to split at the right amino acids, between 118 and 119. The choice of TEVp was for its high specificity and the possibility of splitting it into two halves that could complement each other to regain catalytic function.


Source

The protease originates from the Tobacco Etch Virus.

References